Tyler LeBaron Presents on Hydrogen Health Benefits at AMMG Medical Conference

 

Transcript:
I'm glad I had the opportunity to be
here this wonderful sunday morning it's
bright and early and one of the earliest
things there water development was
hydrogen the first element of the
periodic table and it's that's perfect
right for our talk first off what is
molecular hydrogen well here's the
molecule here it's two hydrogen atoms
that are bound together to form the
molecule molecular hydrogen it's the
Hindenburg it's the hydrogen gas it's
it's we have talked about hydrogen gas
is an alternative energy source for many
years using fuel cells it's three times
more energy dense in gasoline so that's
where most of research of hydrogen has
been however recently hydrogen gas been
shown to be therapeutic and medicinal
and I I came across this whole hydrogen
gas area about six years ago or so and I
was really astounded and I wanted to
learn more about and I started doing
more research more reading more articles
just going through everything that I
possibly could I was an undergrad my
degree was biochemistry I stood continue
reading all those articles I got more
and more excited about it I did a number
of just research projects here and there
and then for my internship to complete
my degree in biochemistry I have the
opportunity to go to Nagoya University
in Japan and as a big deal because
Nagoya is is the fifth most prestigious
university there in Japan it's very well
recognized and I had to prepare to go to
go over there in research with some of
the brightest minds in the world of
academia and my mentor dr. Kinsey oh no
he is the vice dean at Nagoya University
in the Department of neurogenetics and
it's very interesting how he got into
this whole area of hydrogen gas and his
story is basically he heard a lot of
anecdotes and and other colleagues
talking about this whole hydrogen gas ID
and his possibility that could be
medicinal in therapeutic and he being
rather skeptical and a very respected
scientist in his field of neurogenetics
and all the things he's done
the Mayo Clinic and other places
throughout his life he he didn't quite
want to do the whole hydrogen gas thing
because it didn't see his very skeptical
Elmo sound a little like pseudoscience
or something and so what he decided to
do is sued an in-house study where he
was going to see the effects of hydrogen
gas on Parkinson's disease so he he took
wrath and injected him with a toxin to
induce a Parkinson's disease in them and
then he had known in a controlled trial
he had some of the rats drink water that
contains dissolved hydrogen gas and of
course the other browsers drink normal
water what he saw changed his research
career because he saw that the rats
drinking the hydrogen-rich water they'd
had totally totally totally prevented
the development of Parkinson's disease
so when he saw that with his own eyes in
his own lab that totally change research
career and now he has focused entirely
on trying to elucidate what the
underlying molecular mechanisms and
primary targets of hydrogen gases and
that's also my primary desire a goal or
emphasis is what interests me the most
is how is hydrogen gas working because
you see in cells tissues and animals
humans all these different things about
hydrogen gas but the question is how is
it actually working and those answer
remain elusive so in this presentation
I'm just going to briefly give you an
overview of hydrogen gas and its medical
applications what we've seen so far how
it can be used and and that should help
you in your practice and how maybe you
can use it so here's some of my my
objectives here basically the basic
pharmacodynamics of hydrogen gas
including its role as an antioxidant and
it's signaling modulating effects
therapeutic and potential of molecular
hydrogen medical and clinical
applications and the shortcomings of
weaknesses of conventional antioxidants
and how hydrogen gas can overcome them
so right now molecular hydrogen has
shown to have therapeutic potential in
over 150 human and animal disease models
the last count has asked it closer to
170
animal disease models and essentially
every organ of the human body with
displaying the pancreas the brain the
heart the testes the placenta and we'll
talk about more of these in detail the
number of research articles on molecular
hydrogen are also going exponentially
really started about 2007 and the reason
why is because you know that there's
lots of journals and more and more
popping up these days but journals as
you know in the peer review are tiered
at higher levels and this is generally
based on its impact factor in 2007 there
were about 50 articles that were
published on the medical effects of
hydrogen gas and its potential as being
a therapeutic but no one really took a
lot of interest that one of the first
articles is in 1975 by bailey university
in texas am showing that hyperbaric
hydrogen kid has a marked regression of
tumors of melanoma tumors in in mice and
there's a couple of the articles here
and there but not a lot of interest and
really when you think about it how do
you really apply hyperbaric hydrogen to
normal patients hydrogen gas extremely
flammable it's a safety issue so it
wasn't really practical and what
happened there were only 50 articles or
so around two thousand seven but in 2007
those a groundbreaking article published
in this in the journal Nature Medicine
which as you know is one of the most
prestigious highly respected journals it
has impact factor in around 30 or so and
when the article was published in there
which has been cited hundreds and
hundreds of times that really took the
biomedical research area and scientist
by surprise and now researchers just
grown exponentially to now there's
actually around 600 different articles
that discuss its therapeutic application
it's not hit just in Asia but also here
it's going in the US number of these
universities and organizations are also
doing hydrogen research IEC NASA there
there some of them are actually looking
into using hydrogen rich water you can
take the gas to put it into water and
the astronauts can drink that to protect
them from the radiation damage during
space travel lots of easy uses
annulment Linda University this is an
article that was published in 2013 by
dixon and colleagues they say that they
hydrogen has mark therapeutic potential
to help with the top eight out of the
ten a fatality causing disease is listed
by the CDC it's remarkable what we're
finding so far so the question then is
how does hydrogen exert these biological
effects like I said it has this
therapeutic potential essentially every
organ of the human body but how how's it
doing that right well one of the
hypotheses or theories that we've found
so far is that hydrogen neutralizes
toxic radicals is a therapeutic
antioxidant and this again is in 2007
this is the article that I mentioned
earlier those published in Nature
Medicine you can read the title hydrogen
acts as a as an antioxidant by
selectively reducing cytotoxic hydroxyl
radicals and so they made the high of
course hydroxyl radicals via the fenton
reaction against see reduction and it
was very very very profound what they
were able to see but but note the word
selective because we're going to talk
about that first off what are free
radicals just we understand these are
free radicals have the unpaired electron
they're very reactive then you also have
the reactive oxygen species like
hydrogen peroxide s and a number a
number of other ones that is very strong
oxidants that can cause damage which as
you see here they can damage RNA DNA
protein cell membranes the link to cell
death and really every disease there is
neurological disorders inflammatory
disorders cancer diabetes and the list
goes on and on how are these reactive
oxygen species generated primarily in
the mitochondria primerica complex one a
complex three electron transport chain
you have oxygen reduction to superoxide
and then of course that can go on to
form hydrogen peroxide hydroxyl radicals
and other oxidants and pro oxidants
which caused all these damage but notice
most of them is in the mitochondria
inside of the cell now importantly the
body as you know has it's our own self
defense system we need oxygen to breathe
I mean how many of you breathing right
now well you do it because you need it
be every time you breathe oxygen though
around two to four percent of that
oxygen can actually turn into these
cytotoxic oxygen radicals that can in
turn be damage to your body so we have
to protect our bodies from this so we
have various peptides and enzymes to
help protect your bodies as you know
things like glutathione superoxide
dismutase catalase the number of other
things what what what we're showing
though is life in reality is balanced
between oxidation and reduction I mean
you can even consider it like a battery
and negative and positive charge life is
totally balanced on this on the one side
you have oxidation you have all your
different things from your your
hypochlorite your of hydrogen peroxide
superoxide and your your nad+ nadp+ all
of these different things is all
oxidants of the body needs and then on
the other side you have reduction and
the whole body everything is balanced
right between us or you can have
oxidative stress or reductive stress and
it's interesting the the molecules that
seem to characterize oxidation reduction
the most is actually oxygen and hydrogen
and when the to react together you form
the life-giving solvent water so it's
kind of romantic story when you consider
all this and the fact that we've been
focusing on oxygen all the time but
maybe missing this this is the yin and
yang type of relationship with the
hydrogen and now the research is coming
back in saying hey this hydrogen is
actually fairly really good for us when
we with via a gene and certain diseases
toxins some of these things may actually
lower our body's own antioxidant
defenses and so maybe we'll have lower
glutathione levels at lower catalase
levels of different things don't have an
excess amount of oxidative stress in the
brain the liver or other organs of the
body causing various diseases now
unfortunately please note this this is
this is a misconception that all free
radicals are just toxic bad kill you
take a million loads of antioxidants and
you're just going to live forever this
important because free radicals or
reactive optional species are very
important signaling molecules the you
you see there they're important for
signal transduction here's a little
pathway you can memorize and you'll be
tested on the later
you have it is important for immunity
vasodilation nitric oxide and people
talk is nitroglycerin viagra all all of
these things are all about nitric oxide
well you realize nitric oxide is a free
radical what a shame would it be for all
of us if we neutralized nitric oxide we
lose a lot of a lot of benefits also the
activation of transcription factors so
importantly like I said the life is
bouncing oxidation-reduction we have to
keep that balance now consider this this
is a review article perhaps this is why
clinical trials with supplemental
antioxidants often have deleterious
effects as this free article clearly
states antioxidant supplements do not
possess preventive effects and may be
harmful with unwanted consequences to
our health now please note there are
certain cases where high doses of maybe
my vitamin C here good a thigh on their
this or that can be very effective but
in the right circumstances in the right
cases and really a case-by-case basis
but when you really look at the
literature you'll see that for most part
when you're taking high-lows
antioxidants it doesn't really offer the
benefits you think it ought and there's
a few reasons why one of them may be a
scavenging too much of it and maybe it's
not actually getting to where it needs
to be in the first place exercise we
know exercise is medicine that has been
the cry of this last few years exercises
medicine well guess what when you
exercise your breathing more oxygen
unless you hold your breath when you
breathe and I wouldn't recommend that
although you do get winded faster the
fact is when you exercise you produce
more free radicals and these free
radicals reactive oxygen species are
very important for your training Bend
benefits in increased model condrey
abiogenesis increase factor
vascularization all of these different
things really can be mediated by these
free radicals and guess what
conventional antioxidants may actually
negate that benefits of exercise
training they've shown studies people
take in high doses of vitamin C for
example that can blunt the improved
insulin sensitivity after a bout of
exercise or maybe a 10-week exercise
program with endurance training
all of a sudden they don't have a higher
vo2 max or they don't have as a higher
as much as the mitochondrial biogenesis
they ought to have because they're
taking so much antioxidants so all of
these things are very important to
consider with with patients with chronic
diseases who may be suffering in their
cells oxidative just consider this
consider this that in the cell there's a
new article published i think i think in
science that showed that you can have a
cell that in one compartment say that
the endoplasmic reticulum which you know
that that's important the ER is
important for protein folding right and
and protein misfolding is like what is
the cause of aging everything well guess
what as you age the ER endoplasmic
reticulum actually loses its stability
its oxidative power its oxidative
potential it doesn't have enough because
it's not as much oxygen potential so
again we have to have that oxidative
potential so in the in the in the ER
we're having not enough oxidative
potential but in the cytosol we're
having too much oxidative potential so
in the exact same cell we're having a
dysregulation of redox homeostasis see
the issue so the question you all have
is will then Tyler why are you trying to
talk about hydrogen gas than antioxidant
if antioxidants aren't so good for us
well that's a good question on the
answer that first off notice the article
it's selective selective antioxidant
it's really only going to scavenge the
most cytotoxic or cell damaging free
radicals such as the hydroxyl radical
okay all these other ones superoxide any
radical look that that's not very
reactive it's going to be regulated by
the body it has a and superoxide
dismutase that'll daedalus mutate that
to form hydrogen peroxide and then you
have hydrogen peroxide well that's not
very reactive either and there's
actually aquaporin channels I hydro
peroxide goes into does signaling things
and then catalase of glutathione
peroxidase takes care of that all these
things are regulated controlled by the
body hydroxyl radical on the other hand
are produced and just kill producing
killed that that's what they do that's
why they live and die they die killing
you so let's not do that let's scavenge
those things with hydrogen gas
and this is the other thing even if
we're scavenge maybe too much hydroxyl
radicals let's even look let's go let's
do some physics and chemistry here look
at the rate constants typical radical
reactions are on the order of 10 to the
10th you see that Joxer radicals to form
hydrogen peroxide ten to the tenth the
rate constant all these ones are about
ten to the tenth notice the last one
hydrogen gas to Ford did against the
hydroxyl radical is 10 to the seventh
three orders of magnitude lower and plus
the concentration of hydrogen gas in the
cells is often very low the micro molar
amount so even at that you're not going
to be scavenging an excessive amount of
these important biological signaling
molecules but it can do it under the
right circumstances such as when there's
oxidative stress not just oxidative
things going on because again that's
that's key and essential to our whole
bean now this is a very important slide
I'm going to talk about each one of
these points please note what hydrogen
gas is and how it's more beneficial than
than your conventional antioxidants
first off we have rapid diffusion
hydrogen gas is the highest diffusivity
of all gases really of anything okay
consider the fact that it's a smallest
molecule in the universe is just two
hydrogen atoms two electrons and two
protons it's neutral so therefore its
hydrophobic well we know that if its
hydrophobic or neutrals can be able to
get through the cell membranes very
easily as opposed to other antioxidant
maybe our are lipophilic its and there's
no by-product is just waters you know
the hydrogen gas reacts hydroxyl
radicals and the by-product is water
we're talk about that a little more
detail let's look at this slide here we
have the the big red thing there that's
the hydroxyl radical and then we have
the little blue dot that's hydrogen gas
notice where those big bang little blue
red things are those are inside of the
cell the nucleus the model Andrea okay
that's where those are at so that's what
we have to get those antioxidants well
let's look at the let's look at the blue
diamond those are hydrophobic
antioxidants if they're hydrophobic
they're fat soluble how that therefore
is going to be a little harder for them
to get in speed in the cytosol of the
cell
where it's more more more of this
aqueous lip water-soluble things because
it's so the thing like vitamin E that's
going to be where in the cell membrane
it's not going to want to go in to say
the nucleus or or or go all the way into
the model andreea you see the problems
so therefore it's not really going to
get to wear these hydroxyl radicals are
being produced in complex 1 or 3 or in
the TCA cycle of succinate dehydrogenase
or things the other problem is
hydrophilic antioxidants okay so how you
going to go through the cell membrane
because number that's made out of fats
well now we have to have various
transporters and all these different
things indeed changes the whole rate
constants of again and given the the
vitamins to to the radicals where the
problems are actually taking place so
now maybe we can see where we're having
were causing reductive stress in some
places and a continuation of oxidative
stress and other compartments whereas
hydrogen gas small molecule neutral its
hydrophobic is neutral so it can diffuse
through all these subset of their
compartments and membranes the
blood-brain barrier tesys barrier
placental bear all these things very
easily where it can actually react with
these hydroxyl radicals and convert them
to water which is at the last thing no
that's the by polishes water think about
it when you have something like vitamin
C vitamin C donates its electrons it's
not vitamin C anymore it it it's it's an
oxidized form right and so now the body
has to rejuvenate that and which should
be used up nadph equivalents or
glutathione or other things it's got a
metabolize it's got eliminate it's got
to do something or else now that could
be a potential toxic pro-oxidant or of
some by part where the bars gotta clear
it right versus hydrogen gas it's going
to react to these hydroxyl radicals in
the byproduct is simply water I mean
some people even drink that stuff now
importantly though hydrogen gas does
reduce oxidative stress because we're
talking about all these things about why
hydrogen gases selectivity the rate
constants well then does it really work
and and what I did is like I said
there's Robert you know six hundred
articles now I've read every single one
of them and I've gone through and I've
done all this stuff and I just took some
of the neat things and I kind of made
prepared this table so here we see
markers
box of distress this table comes from
from from cells from tissues studies
from from animal studies and human
studies and this is where hydrogen gas
can do his thing in the right conditions
in the right cases remember if your if
you have normal MDA levels which is mel
and aldehyde routes and marketer box of
distress hydrogen gas not going to
reduce it it's not going to do it
because it's a mild reductant and you
don't want to reduce oxygen distress
more than you need to okay so mocks
locks in stress these are very common
markers md80 bars 80 d HD g the marketer
DNA damage all these different ones to
hydrogen gas showing a can reduce
oxidative stress on the other side check
that out markers of antioxidant status
hydrogen gas actually able to increase
or upregulate to bring back to
homeostasis our body's own antioxidant
enzymes such as superoxide dismutase
glutathione catalase glutathione
peroxidase glutathione s-transferase
with on reductase all these things
hydrogen gas also helps to do which
we'll talk about here in a little bit as
well now there are three ways hydrogen
gas is able to do they do this to reduce
the oxidative stress one of them as i
said before hydrogen gas and they
actually be scavenging these hydroxyl
radicals as i've shown in the nature
article and again you form water another
one is hydrogen gas can actually
activate what's called the nrf2 an earth
to pathway which is an app it's in that
transcription factor right it hold
antiox response element part of the DNA
hydrogen gas can activate the inner tube
pathway which leads to an increased
production of superoxide dismutase
glutathione catalase and subsequent
induction of human oxygenase which again
is extremely sad a protective and
somehow that does it notice i have a
question mark that is what intrigues me
i want to know how is it doing that okay
what is the primary target some kind of
a higher so if requested here it's gonna
your cell membrane volume type thing is
going to attach to that and do this
little magic it's it's amazing you know
being what you know i was at nagoya it
for a few months doing research there
and so they able to actually research
some of these signaling pathways and
whatnot and seeing how hydrogen gas is
actually changing the different levels
and I'm like okay how's it doing it how
is this doing it just it's mind-boggling
it's awesome okay so
go going on hyejin may also prevent this
excessive amounts of reactive oxygen
species by actually preventing them
perform in the first place via cell
modulation here we have like a knock
system and NADPH oxidase on the one side
we see it's activated when it's
activated the complex is glued together
it's not really glue super glue you know
we don't pad that in ourselves but for
the sake of this they're stuck together
and when it's activated like that we're
reducing oxygen to form superoxide and
the superoxide can go on a form various
other radicals that are very damaging
and when that's heightened when that's
when that's a activated via inflammation
or various pathologies or diseases we're
going to have more and more oxidative
stress maybe dr. stress somewhere else
and the whole again dysregulation of
redox homeostasis well hydrogen gas in
the right circumstances can actually
down regulate this nadph oxidase system
thus preventing ross formation in the
first place now don't we say prevention
is better than the better than treatment
well here's hide hydrogen's it's a smart
guy you know it's a simple humble but
you know this is dealing with what it
does best now importantly though the
antioxidant activity may not fully
answer why is to have so many benefits
because the fact is when you chain you
take hydrogen gas well you're going to
excel most of it out of you out of your
breath it's not going to be in your
system for you know days and hours and
whatnot it's going to be cleared very
rapidly so why isn't then you can take
hydrogen gas through you know drinking
hydrant water or inhalation or something
and yet still have the cytoprotective
effects hours or even days later this
residual effect because if hydrogen gas
is not even in the cell then how is it
scavenging those hydroxyl radicals right
so there has to be something more to
this and this is again where I get
really excited so bear with me if I
start doing you know jumping up and down
first off h2 may actually acting is some
sort of novel signaling molecule and the
reason why is the extended the right
conditions hydrogen can alter the levels
like tis of over 200 different
biomolecules I got two and a bottom
office I just went through a bunch of
articles and like oh okay that's an act
that's a transcription factor that's a
protein
and this is not an mRNA because you do
you know rt-pcr or something you have
different you know mrna levels or
whatnot that doesn't actually account to
like your western blot of a natural
protein so these are all things that
have been altered on the protein level
which is what matters and hydrogen gas
able to upgrade glitter to down-regulate
or 200 these different things how I
don't know but when you compare the this
hydrogen gas because it is a gas to
other gas the assuming molecules and
this is very interesting when you let's
talk about nitric oxide nitric oxide it
was a silly molecule but before that in
80s or 70s and this this time they knew
that there was this vasodilating active
thing as Kabul endothelial in the seal
of a vasodilator and and they didn't
know what it was I just knew it was some
agent somebody proposed that it was a
gas nitric oxide and what happened he
got laughed at and you know he ridiculed
and everything because higher gas can't
do that nitric oxide no way no way and
guess who won the Nobel Prize right 1998
he got the Nobel Prize for showing the
es indeed it was i guess it was doing
this and subsequently found other guests
silly molecules such as carbon monoxide
which is very toxic at high levels but
extremely therapeutic and lower doses
and our body actually makes carbon
dioxide through a team won and different
things but we see here this is my little
graph i prepared for you if this this is
why it's so elusive when you consider
hydrogen sulfide that you can see the
chemical structure is polar we know the
mechanisms s of hydration protein to the
Sistine etc we have nitric oxide was
very polar it's a radical and we know
its mechanism notch oscillation it binds
to the iron on the guanylyl cyclase
higher monoxide you know one of the
targets like hypoxia-inducible factor 1
alpha human oxygenates we see these
mechanisms we see the chemical structure
how it's going to interact with these
different moieties of this is a cell or
different things however hydrogen gas is
nonpolar it's neutral so how is it
working what are the mechanisms and as
you can see there that's how it's
working these ? city when you have a
very good microscope and you're looking
to start to see these question marks in
there that's what I feel like sometimes
so really this is what it is we have had
a guest doing this who knows how many
pathways or whatever is doing or maybe
it is just you know master regulators
and drivers that are then in turn
inducing all these things so hydrogen
gases is altering these things
indirectly not directly which ones that
are affecting directly I don't know
we're getting closer and I just had a
talk with a Japanese scientist to a few
days ago talked over two hours and he is
some studies you've been doing I we
can't disclose it at this point but
we're getting closer and it's it's
exciting i'm getting ready to jump a
little bit so i'll just move on before
okay so the fact is we know that
hydrogen gas is working we've seen tons
of studies it's awesome it's amazing we
know what we don't we don't know how it
is working and I have an idea maybe you
know it's a romantic story and you know
we like romance and stuff so I decided
to give up maybe here's a reason why
it's working and and here it is perhaps
the reason h2 exerts a biological effect
is because h2 has been intimately see
it's romantic intimately involved in the
origins of the universe the genesis of
life and the evolution of eukaryotes so
you plant animal cells check it out so
the very beginning hydrogen was involved
in order to the universe and one of the
famous astronomers he like a Harlow or
something he said if God if God did make
the world in one day that word would
have been hydrogen I said that's
interesting he considered this whole
thing about hydrogen it is the father of
all the elements you know the Big Bang
14 billion years ago and then we get
hydrogen and all the other molecules
that are or elements with periodic table
so we should think hydrogen for that
then we have the next part hydrogen was
involved in the genesis of life we even
go back to the famous Stanley Miller
experience and in science back in
nineteen fifty three hydrogen gas is one
of those molecules there that was
involved in the production of the
various base and molecules of life and
the hydrogen gas could count from this
you know reaction with iron a hydrogen
sulfide from hydrogen gas we have this
picture here which is also shown here
deep sea hydrothermal veldt's vents from
that from the ocean where life likely
originated from will actually may have
served as an energy source to these
chemo little autotrophs that hydrogen
gases you know has these electrons very
energy rich so these enzyme complex
whatever eventually could develop the
ability to extract those electrons out
this hydrogenase activity forming the
first life the first types of
prokaryotes and whatnot and very
interesting an article in science 95
said the last common ancestor of life
also metabolized hydrogen for energy
that's again suggesting its involvement
in this entire evolutionary process this
is interesting many of us heard of these
you know natural healing waters and all
this kind of hocus pocus stuff actually
some of these waters that have been
documented to have potential therapeutic
benefits and when you go back to the
waters and some science have gone back
then they've actually tested these
waters to see what's in them and guess
what they found hydrogen gas mahajan gas
could be in the waters via maybe
anaerobic bacteria or base all catalyzed
reaction from the various metals I'm in
the water but some of these waters
contain small amounts of hydrogen gas so
it's pretty interesting and now let's
talk about how natural or safe hydric
acid is for a little bit the fact is
every time we eat our fiber and our
meals and our foods and vegetables are
the bacteria in our intestines and you
know you know the story bacteria is like
extremely important it's like a been
going on for decades now about how
important to bacterias versus microflora
one of the reasons why is because of
when you eat your fruits and vegetables
or fiber or not a dispro carbohydrates
you're producing hydrogen gas and you're
getting as much as 10 liters of h2 per
day and you had a higher breath ppm and
so unique so therefore you have
micromolar levels of hydrogen gas so
therefore you have a basal level of
hydrogen gas in your blood since the
dawn of time if you're alive back then
now this is a actually so hydrogen gas
from your bacteria does indeed have a
therapeutic effect in fact in 1988 based
upon the redox potentials on the Nernst
equation hydrogen gas was suggested to
be and
the Occident from the bacteria and in
2009 it was confirmed by report from the
foresight Institute in Boston
Massachusetts and the University of
Florida this is an awesome study all
they did is they took these ghouls just
you know rodents or rats and they and
they of course have bacteria that the
e.coli with the what's called the
hydrogenase enzyme that could produce
hydrogen gas and when they gave
lactulose which is a non digestible
carbohydrate the bacteria metabolizes
that lactulose produces an obscene
amount of hydrogen gas and all these
benefits are seen you know protection to
the liver well they took these the coli
they did a genetic knock out so they
remove the genes that produces the
hydrogenase they put the bacteria back
into the rats and then they gave la
chula so now the bacteria still has the
rat the bacteria has the rats that'd be
interesting the rats have the bacteria
and they give the the fiber to them so
you'd think you know okay they still got
the bacteria so they should be fine and
guess what the therapeutic benefits are
like negated eliminated gone it's like
no that's interesting what when they put
the bacteria but when they put bacteria
back in that had the hydrogenase ends
i'ma give it like she lost all those
benefits reinstated that's suggesting
that again hydrogen gas is the prime is
maybe a primary method or a reason of
why fiber so good for us okay this is
this is crazy this is awesome this is
from the nastiest publication of
physical little chemistry letters um
this is hypothesis based upon some
physics and whatnot but actually
resveratrol we've taught heard about it
is very good for us nobody really knows
the exact molecular mechanisms of why or
how it works but there was an article
that suggests that maybe the benefits of
resveratrol are actually mediated by the
production of hydrogen gas because in
the motto con dria you have electron
leak and the electron they can donate a
lecture on to the two parts of the
polyphenol groups the resveratrol and
they have disassociate electron
attachment and therefore you have
production of hydrogen gas which can
then go and scab and hydroxyl radicals
right they were to be informed so in
there's a number of other articles that
are coming out the old and that's the
case and this one could work too because
this you do the calculations and they
hate this make sense so again this this
this is kind of groundbreaking stuff
here so method administration
we have you know of course you do
hydrogen-rich saline so you have saline
and you have the school with hydrogen
gas in there and you can done Studies on
that ingestion of hydrogen rich water
where you can simply bubble hydrogen gas
into the water which isn't too practical
there isn't there's a method to get Thea
like electrolysis they have hydrogen
producing tablets they're made of
metallic magnesium so not like a
magnesium chloride assumption but a
national metallic my knees in react to
the water to form magnesium hydroxide
and hydrogen gas and met many people
have been using that with remarkable
benefits let's put the tablet in the
water and people can do like a one tell
the day daily maintenance and for you
practitioners if you have looking for
somebody with pathology whatnot they can
take another one or two or three or four
the dri my knees IAM is you know 500
milligrams and mostly tablets contain
around fifty fifty five milligrams of
magnesium or so so you just put it in
the water if it after vest has produced
a ton of hydrogen gas and the patient
can drink that and you start seeing a
lot of benefits so it's pretty easy to
administration and then of course
there's inhalation of hydrogen gas you
know that that handsome guy is not that
handsome but who's judging but it is me
i I lectured in I gave a talk in Beijing
at the biomedical hydrogen symposium no
actually I was there earlier in Shanghai
that's where this was taken at I was
made just with the company they wanted
to talk about hydrogen research and
different things and they they have this
machinist is the big daddy you know and
they have these various medical clinics
there in China and people go in there
and they inhale the hydrogen gas and
it's it's it's amazing the things they
have they have found that I mean they
have they've collected over 20,000
patients worth of data case study after
case study after case study I met a
woman I saw a video of her first and a
whole the whole thing about her but then
I actually met her when I went to do to
do my inhalation therapy because i'm
actually 55 years old if he didn't know
but I've been doing hygena for a while
so when i went to do my am I supposed to
do this inhalation and I got to meet her
and what happened with her is she she
got a stroke and that's the unfortunate
part but there's good news she had the
stroke they had their brain imaging and
it was just totally messed up she was
actually paralyzed like
lot in her left side of her body can
really move her left hand can walk you
know or do very much stuff immediately
started hydrant inhalation and within a
few days later that week she regain
mobility back she could walk up the
stairs all by her so she can move her
left arm and there's case after case of
the case of people who are like in a
coma ready to die take him off oxygen
all of a sudden put them on hydrogen
inhalation therapy and they wake up a
few hours later start doing better I
mean it's just like I don't even believe
this stuff but it's like right here and
there's so much empirical evidence I'm
like okay this is awesome they I believe
that the Chinese FDA just gave the FDA
clearance the medical approval so they
can sell to to medical hospitals there
in China so it's its enormous all the
amount of research things that are going
on at very high levels the now now let's
go to just some of the study so you can
see what's what word of the study is
actually showing and first I wanted to
compare the superiority of hydrogen to
other antioxidants there we have the
long term treatment of hydrant saline
debates testicular oxidative stress
induced by nicotine and mice may know
it's an epidemic people using tobacco
smoking and that causes the testicle
problems and if you if you want those
and save them then this is a good option
for you if we compare this to other
antioxidants so this is very powerful on
this far graph we have tea with stands
for Tyler no sense for testosterone
first we have the control and that's
that's the normal range you want to be
at and then you have no treatment right
but you get the nicotine and you notice
that just plummets it right down it's
like what it's like you'll see these
things and they always get stars and
like that's like contradictory because
then I was in school I got stars on I
did good things so so it plum is that
right down and then when they give
hydrogen gas it just brings the back up
and you know no star for you maintains
that hydrogen gas that the testosterone
levels rather they need to be but when
you gave a vitamin C vitamin E there was
no statistical improvements same thing
with sperm motility and sperm
concentration again was a hydrogen gas
show the superiority of this similar
finals was found with the effects of
vitamin C and vitamin E and hydrogen on
the placental function and Joe hablas so
people have preeclampsia
for example the hydrogen gas was shown
to have a beneficial effect on HCG where
vitamin C vitamin E did a little bit as
well however if you look at the other
you can go and look at this article but
it was shown that vitamin C and vitamin
E actually suppress cell viability so
they're like not live in as well and
increased you know immune responses
tumor necrosis factor alpha and other
problems whereas hydrogen gas didn't
have any of those side effects it just
had a beneficial effect on HCG this is
very interesting cuz radiation is a big
talk you know all the damage has come
from this i mentioned like NASA with the
astronaut that's an interesting concept
here we're showing the concentrations of
800 HD g which is a mention of the
marketer of DNA damage this is really
cool when you look at this this first
graph there's hydra where did you have a
marker you know your your about what
point seven or so when you give that
that's where you normally this normal
people right there's no hydrogen gas is
given no that's actually an animal model
but normal no hydrogen gas is given no
radiation is given and that's the normal
level if you will about marker right so
you always have just like small amounts
of inflammation that you need to have a
little bit of this so that's where that
was when you gave hydrogen gas and no
radiation look how much hydrogen gas
affected that not really anything at all
do you see the benefit in that you
notice when you take like maybe other
pharmaceuticals or drugs or just
different things and then that just is
going to go in there and just decrease
it decrease it or increase it increase
it well that could be bad because we
want to maintain homeostasis hydrogen
gas really doesn't perturb homeostasis
is if it's where it needs to be it's
going to stay there and that actually is
one of the reasons why hydrogen gas is
so difficult to study with many drugs or
things you can take your cell you throw
the the drug or the compound in there
and then you can do your study and you
can see how that affected the cell and
in your in vitro system or something
where the hydrogen gas is a little more
complicated kids you can't as easily do
that you almost have to induce some sort
of toxin or some salt to it or something
and then see how hydrogen gas attenuated
and mitigated those effects so you have
all these more variables to look at
because hydrogen gas really does not
dis regulator perturb the normal
homeostasis or not when it should not be
or and because of those things that is
why one of the reasons why it's how
difficult to to study the molecular
mechanisms of hydrogen gas but
importantly this is what you really want
to know is that really big graph that
that's when you gave the radiation so
now we have tons of DNA damage and the
person's dying is probably already dead
but when you gave the hydrogen gas you
see it just it just decreased that is
very significantly this is this hydrogen
gas really has a very wide breadth of
uses of course for athletes as well
here's a pilot study on the fix to drink
and hydrants water and muscle fatigue
caused by acute exercise and elite
athletes this is very interesting they
actually showed you know um I had the
beneficial in the fatigue they showed a
less lactic acid or lactate there's not
really a lot to Casa de thing we're
gonna get into that but the thing is I
showed a decrease in lactate not the
lactate is bad but what that shows
remember when you when you make ATP use
of glycolysis we use the model quantity
the electron transport chain model
counter produces a lot more ATP when
your exercise really hard you start
producing all these free radicals all
those free radicals from a damaged
mitochondria compromised and I have less
ATP of value mitochondria so maybe need
start relying more on glycolysis and so
now you can start producing more more
acid the hydrolysis of ATP and
glyceraldehyde 3-phosphate and different
things now your house are going to
increase difference is going to happen
and now you have more a lot tate is
going to be produced as a logic acid but
which really lactate it's going to be
produced and that's what you see so what
hydrogen gas is showing that there's not
as much lot tate production that's that
maybe because hydrogen gas may actually
be stimulating the model andreea and
protecting it from being damaged so now
you can maintain normal ATP levels now
you can still rely on model contra 4 ATP
needs instead of your you to glycolysis
and of course and you consider the fact
that how important ATP is for everything
ATP as you know is the energy currency
this
you put you put the needs of ATP in what
we really need you you need about the
same pounds of ATP the e-way so if you
weigh 150 pounds you you you need about
150 pounds of ATP per day that's how
much is resynthesizer millions and
millions of mitochondria and end your
cells that's a lot and in a serious
athlete to do in like a marathon or
something they need they mean they may
need twice that ATP so up to 300 pounds
of ATP okay so the model counter plays a
very large role so being able to protect
the mitochondria is extremely important
and in fact that we see here hh2
stimulates mitochondrial function this
is an allograft this cardiac allograft
article but it's very good grass we're
going to show this a complex one two
three and four and five all of these
things you know with an electron
transport chain so in hydrogen gas can
actually stimulate them in fact even
beyond control the control in some cases
which when you stimulate these complexes
you're going to have higher ATP levels
which shows again here you see even
above control you have higher ATP levels
model Kandra does seem to be a major
place of hydrogen gas is exerting some
of these therapeutic benefits this this
if this is a very powerful study this is
this is pretty cool the maternal
molecular hydrogen administration
ameliorates rat fetal hippocampal damage
caused by uterus ki mera perfusion okay
so when the mother is delivering the
baby sometimes you can have an IR injury
or different things and that's gonna
cause a lot of a lot of brain damage to
to the to the infant and that could be
very very sad and very damaging so look
at these graphs here we have the Sham
and that means you know the delivery
went perfect everything was totally fine
and then you have the IR the scheme your
perfusion injury and you notice on the
x-axis the degenerated cells are just
you know very high they just really go
up there because you have this IR injury
now then you can have the mother drink
hydrogen the hydrogen rich water because
you can't really have the baby drink
because the stomach and it'd be kind of
awkward
so the mother is actually drinking the
hydrogen-rich water and maybe because
again hydrogen gas is so small it can
just penetrate the placenta and
everything very easily but look what
happened when the mother drank the
hydrogen-rich water and the ir injury
was given and made it as though it's
like the ir injury never happened and
when I saw that I was like yeah I don't
believe it but actually you see the
third author on ok get skin giono and
he's my guy in Nagoya University and I
trust what he does immensely so I was
like okay it must be true but I'm still
gonna call him on it it's it's a
remarkable some of the things that we're
finding this is again just I'm going to
go through just a couple abstracts you
know look at the title a little bit a
hydrogen supplemental EIR so again we
have air inhalation I actually can
reduce the changes of pro-oxidant enzyme
and gap Junction protein levels after
transient global cerebral ischaemia in
the rat hippocampus so again back to the
neurological issues this this one here
showed that had the expression of cox-2
and the connections and proteins were
actually up regulated well the neural
nitric oxide synthase which can cause a
lot of damage if that's too too high I
was actually down regulated after three
days of after the injury that was given
so hydrogen gas again helpful in this
area molecular hydrogen drinking water
protects against neuro degenerative
changes induced by traumatic brain
injury this is from the University of
Washington and Wisconsin couple
organizations this is an amazing article
it's almost ill kind of difficult to
believe but these are pretty good
researchers but they found that hydrogen
gas is really can with protective on the
mitochondria and the ATP production and
actually they suggested that hydrogen
gas could actually increase ATP levels
via what's called the Jag and rafa
reaction which is interesting it's in
the model kanji doesn't really rely on
the electron transport chain with like
we don't need to go into that but it's
it's pretty interesting what we're
finding here the oral intake of Hydra
bridgewater ameliorated chlorophyll
erous induced neurotoxicity and rats
that's just an organophosphate you know
a
a normal pesticide and the US a big deal
these pesticides hurting us all those
kind of stuff and I guess there's some
debate about that but what what we see
those hydrogen gas can actually offer
some protection against the
neurotoxicity caused by this stuff in
this article there was a it did so by
activating the acetylcholinesterase
activity which again is difficult to
believe because that's like already has
like the very high K cap but actually
talked to the researcher who did this
when I was in Beijing and I talked about
her results and she went on about him
she was very she did numerous experience
no but they're they're really going
after this stuff and regardless that was
her kind of mekin how it's working
regard so hydrogen gas was protective in
this area here hydrant Mitchell water
protects against ischemic brain injury
and rats by regulating calcium buffering
proteins so again we have you know this
whole brain injuries and things which
again is a huge issue these days
hydrogen gas very neuroprotective this
is awesome hydrant water ameliorates
amyloid-beta induced neurotoxicity is
like Alzheimer's for example this is
through the up-regulation assert one fox
03 a by stimulation of A&P activator
protein kinase SNK and CMC cells so
that's like awesome because of course
the sirt1 protein is like it's it's okay
i understand if it's awesome huh there a
hydrogen hydrogen water improves
neurological function through the
attenuation of the blood-brain barrier
disruption and spontaneously
hypertensive prone rats so that may be
one of the reason why hydrogen gas is
also effective it can help attenuate the
burberry and we know it's a big thing we
have a disruption in that via
hypertension or drugs or what have you
hydrogen gas i'm able to ameliorate some
of those things oral hygiene water
induces in your protective ghrelin
secretion ghrelin the hunger hormone
right this is very interesting you've
heard about the benefits of fasting
before the intermittent fasting and
different things of this nature well one
of the reasons that fasting is
beneficial for you because you increase
ghrelin and ghrelin
is this a Zacks to the second messengers
extremely protective neuroprotective has
lots and lots of benefits so one of the
reasons fastens get feels because you
have ghrelin well one of the reason
drinking hydrant water is good for you
is because you also enact gastric
ghrelin secretion and I ghrelin intern
acts a second messenger to go and help
different parts of the brain actually in
the Parkinson's disease studies that
I've talked about i'll talk about again
it was most likely gowlan that act as a
second messenger to offer that the
cytoprotective effect of the stanchion
even more so than hydrogen gas
hydrogen gas did so indirectly via the
second messenger ghrelin own heritage
right here pilot study of age to therapy
and Parkinson's disease a randomized
double-blind placebo-controlled trial
this is a 48 a trial they did and
awesome results and of course I've heard
him you know there's anecdotal case
reports of people taking like the
hydrogen producing tablets it'll put
them in the water in people's
parkinson's disease often what I would
say is it's not going to give a reversal
of the Parkinson's but maybe a better
quality of life maybe decrease the the
aging that's going to occur or the the
the downward spiral that generally
occurs in Parkinson's disease however
I've heard quite a few reports now
people who are taken I taken taken the
hydrogen that I have like the symptoms
they you can see they have the symptoms
and then I'll after a few weeks or
whatever them taking the the hydrogen
they totally don't even have that does
that anymore and they wouldn't be
diagnosed as having the Parkinson
disease so I guess in some case maybe it
is possible to have these types of
reversals the hygienist water enhances
the 55 f year which is a chemotherapy
drug inhibition of colon cancer this is
very powerful chemotherapy widely used a
it's very toxic of course to cancer
cells hopefully that's a goal but also
to our ourselves and and first off a lot
of studies are showing a hydrogen gas
can can actually protect the negative
side effects of various chemotherapy
drugs like renal induced not
neurotoxicity some dips and different
things so hydrogen gas the can can
protect against the negative side
effects of drugs like aspirin induce
care
mucosal injury chemotherapy in this
study a hydrogen gas actually offered an
additive effect of the chemotherapy drug
where this is a colon cancer in rats but
hydrogen gas when you gave the drug
there was this much affect him then he
gave hydrogen gas and the jugs as an
additive effect and hydrogen gas alone
could help a little bit and it depends
on the cell line of cancer cancers
there's a lot of issues with cancer
that's why it's called cancer hey this
is a pretty powerful here consumption of
hydrogen which water prevents
atherosclerosis and polio protein Enoch
out my so when they have this genetic
knock out they're going to get off his
closest very easily hydrogen this is
this is part of the other the quote here
the authors in the article state when
they compared these results to other
things the efficacy of hydrogen water
seems to be greater than folic acid
vitamin e iron alpha lipoic acid and
other things in the prevention of this
of this aphid explore OSIS so again very
powerful now briefly again the safety
it's very well established i'm in least
all the cases that there's really no
known toxicity that we have found so far
i mean it it has been you know but
there's definitely hundreds of studies
already just like therapy dick
applications but the fact that he's in
deep sea diving since the 1940s to
prevent decompression sickness because
no legal like nitrogen narcosis and
whatnot hydrogen gas is able to prevent
that from happening and then of course
from our intestinal bacteria like i said
from the dawn of time we've been
producing hydrogen gas we have these
basal levels of hydrogen gas in her body
all the time so the safety profile of
hydrogen gas is enormous and so the
first rule right do no harm and then if
something may work let's try it so I'm
like that's why I'm so passionate about
hydrogen gas is because in reality I
can't really think is something that's I
guess more safe as a supplement that has
therapy to potential than hydrogen gas
and yet has such profound therapeutic
potential so here's a brief summary
again therapeutic potential in 150 or
about 170 and essentially every organ of
the human body neutralizes the toxic
radicals
they selectively antioxidant
up-regulates production of the body's
own antioxidants when it needs to be
again if you already had a little bit of
fun it's not going to increase it more
because I couldn't bad i has a pasta is
possible signal modulating properties
which gains often these antidiabetic
anti-apoptotic anti all the different
things in fact there's one article
published on nature publishing group of
the general obesity showing that
hydrogen gas kid kids activate the
fibroblast growth hormone 21 which
induces energy expenditure for like
weight loss of things they found that it
was in a my study but they found I think
it was a drinking Hydra rats drinking a
hydrogen-rich water had about the same
effect as a 15-percent caloric
restriction and and when then there was
an additive effect when you and you did
both of them so any hydrogen seems to
mimic kind of fasting and what we
already talked about ghrelin that's
again fasting so it's pretty powerful
there too has a very high safety profile
many easy methods administration
probably easiest of course is just
drinking Hydra much water briefly
because I know the question is there is
what's more effective inhalation or
hydrant water well it depends sometimes
inhalation can be more effective but
most of the time why don't know most of
time but i'll say it for right now but i
might change tomorrow drinking hydrant
water is just as effective and sometimes
more effective in fact in apapa study
only the hydrogen-rich water was
effective whereas a continuous and
administration of hydrogen inhalation
was not effective and that could be two
in the need of an intermittent or post
type of exposure as opposed to this
continuous exposure so drinking hydrants
water of course is the easiest and is a
very effective way of getting the
hydrogen gas so this is kind of my
prediction the market is generally 10
years behind the science it's kind of we
season like a lot of different things
out there so 2007 was really the
beginning of focus research on hydrogen
so i kind of predict by two-thirds of
the 17 will be the year of h2 awareness
we're just more of the universities and
people will be like well but let's use
us and do clinical trials there's a lot
of clinical trials that are underway or
starting right now
in mainly in Asia and so when those get
published I mean just really just take
off so here's some references you can
write these down really quick so join
the hydrogen revolution this is my again
the nonprofit foundation luck magic
foundation I will we only have like two
minutes left so if there if there are
like a question or two you can answer
them of course I will be out there at a
table and I'll answer all of your
questions there so thank you very much
for your time and I had a had a pleasure
being here

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